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Reverse-phase high-performance liquid chromatography of proteins: The separation of hemoglobin chain variants |
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| Authors: | Petro E Petrides Richard T Jones Peter Böhlen |
| Institution: | 1. Laboratories for Neuroendocrinology, The Salk Institute for Biological Studies, La Jolla, California 92037, USA;2. Department of Biochemistry, School of Medicine, University of Oregon, Health Science Center, Portland, Oregon 97201, USA |
| Abstract: | The potential of reverse-phase high-performance liquid chromatography for the separation of closely related proteins has been investigated. Using an octadecasilyl silica stationary phase and a propanol/pyridine formate solvent system normal α- and β-chains of human hemoglobin have been separated from several of their respective mutant chains which differ by single amino acid residues only. The results suggest that reverse-phase high-performance liquid chromatography is a powerful tool for the separation of medium-sized proteins with minimal structural difference. |
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