A 59Co NMR relaxation probe of macromolecule anionic binding sites |
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Authors: | T. Raj C.R. Bennett R.G. Bryant |
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Affiliation: | Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455 USA |
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Abstract: | 59Co NMR linewidth measurements are reported which show that hexacyanocobaltate-(III) ion may be used as a sensitive probe of protein interactions with anions in aqueous solutions. Applications are demonstrated to bovine serum albumin where the probe ion binding is monitored as a function of pH and is displaced from the protein sites by hexacyanoferrate(III) ion. The general utility of complex metal ions is suggested as a generally useful approach to the analysis of ion-macromolecule interactions. |
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