Modification in the purification of the sex steroid-binding protein of human serum by affinity chromatography |
| |
Authors: | Philip H. Pétra Judith Lewis |
| |
Affiliation: | Departments of Obstetrics and Gynecology, and Biochemistry, University of Washington, School of Medicine, Seattle, Washington 98195, USA |
| |
Abstract: | A purification procedure for the sex steroid-binding protein of human serum is described. The procedure is significantly superior to that recently published (K. E. Mickelson, D. C. Teller, and P. H. Pétra, 1978, Biochemistry17, 1409–1415) and should replace it for the routine preparation of homogeneous protein in relatively larger quantities. The steps involved diethylaminoethyl-cellulose chromatography, affinity chromatography on 5α-dihydrotestosterone-17α-hexanyldiaminoethyl-(1,4-butanediol diglycidyl ether)-agarose, and preparative polyacrylamide gel electrophoresis. The most important difference between this new procedure and that previously published is the affinity adsorbent with contains the steroid covalently linked at the 17α-position rather than the 17β-position. This modification allows the purification of at least 12 mg of homogeneous protein per preparation with a 63% total yield. The properties of the homogeneous protein are the same as previously described. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|