Incorporation of [2,3-13C]succinate into methyl-branched alkanes in a termite |
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Authors: | Gary J Blomquist Arthur J Chu John H Nelson JGeorge Pomonis |
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Institution: | 1. Department of Biochemistry University of Nevada, Reno, Nevada 89557 USA;2. Department of Chemistry, University of Nevada, Reno, Nevada 89557 USA;3. USDA-SEA, State University Station, Fargo, North Dakota, 58105 USA |
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Abstract: | Carbon-13 nuclear magnetic resonance was used to examine the incorporation of 2,3-13C]succinate into methylalkanes in the termite Zootermopsis angusticollis. Carbons 2 and 3 of succinate were preferentially incorporated into the branching methyl group(s) and the tertiary carbon(s) of monomethyl- and dimethylalkanes. These data support a pathway in which succinate is metabolized to methylmalonyl-coenzyme A, and is then incorporated in place of malonyl-coenzyme A at specific points during chain elongation. |
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