Radioimmunoassay for the detection and quantitation of bruceantin |
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| Authors: | Kei-Lai L Fong Dah Hsi W Ho Carol JK Carter Nita S Brown Robert S Benjamin Emil J Freireich Gerald P Bodey |
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| Institution: | Department of Developmental Therapeutics, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 6723 Bertner, Houston, Texas 77030 USA |
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| Abstract: | A radioimmunoassay for a new anticancer drug, bruceantin, has been developed using 3H]acetylbruceantin and antibody induced by immunizing rabbits with succinylbruceantin-bovine serum albumin conjugates. 3H]Acetylbruceantin was synthesized by reacting bruceantin with 3H]acetyl anhydride. The assay is simple and reproducible. The standard curve was linear on a logit-log plot, and the lower limit of sensitivity of the assay was 1 ng/ml. Using this assay, drug levels were easily determined in tissues of experimental animals following bruceantin administration. The assay procedure does not require sample extraction for plasma, urine, and bile. Bruceantin in other tissues can be extracted quantitatively with ethanol before being measured by the radioimmunoassay. |
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| Keywords: | To whom reprint requests should be addressed |
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