The recovery of transfer ribonucleic acid by binding to DEAE-cellulose induced by ammonium sulfate |
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| Authors: | IC Gillam GM Tener |
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| Institution: | Department of Biochemistry, University of British Columbia, Vancouver, British Columbia, V6T 1W5, Canada |
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| Abstract: | tRNA samples may be adsorbed quantitatively to DEAE-cellulose from 2m ammonium sulfate solution, pH 4.5. Washing with 0.3 m NaCl removes the ammonium sulfate and the tRNA is recovered in ethanol-precipitable form by elution with 1.1 m NaCl. The procedure allows convenient and efficient recovery of tRNAs from larger volumes of aqueous salt solutions. A moderate concentration of sodium chloride does not interfere with adsorption. |
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