Author index for volume 202 |
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Authors: | R.A. Gravel K.F. Lam D. Mahuran A. Kronis |
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Affiliation: | Research Institute, Hospital for Sick Children, Toronto, Canada M5G 1X8 |
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Abstract: | A procedure is described for a simple two-step purification of human liver propionyl-CoA carboxylase. The method is based on acid and carbon tetrachloride extraction to remove other biotin carboxylases followed by an 800-fold purification through biotin-pretreated, monomeric avidin-Sepharose 4B-CL with elution of active enzyme using a biotin gradient. The enzyme had a sedimentation coefficient of 17.4 S and polyacrylamide gel electrophoresis after reduction and alkylation revealed two nonidentical polypeptide chains of 75,000 and 60,000 Mr. The heavier chain was identified as the biotin-containing subunit by electrophoresis after avidin binding. |
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