Separation of dipeptides by high-resolution gas chromatography on a fused silica capillary column after trimethylsilylation |
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| Authors: | Miral Dizdaroglu Michael G. Simic |
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| Affiliation: | 1. Food Engineering Laboratory, U. S. Army Natick Research & Development Command, Natick, Massachusetts 01760 USA;2. National Bureau of Standards, Washington, D. C. 20234 USA |
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| Abstract: | Separation of trimethylsilyl derivatives of over 50 dipeptides was achieved by high-resolution gas chromatography using a fused silica capillary column coated with methyl silicone liquid phase. Excellent peak symmetry and reproducibility were obtained. Several pairs of sequence isomeric dipeptides could also be well separated. Application of this approach to peptide sequencing by means of the dipeptidyl aminopeptidase-gas chromatography/mass spectrometry method is also discussed. |
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| Keywords: | Correspondence should be sent to this author. Present address: University of Maryland Baltimore County Chemistry Department Baltimore Maryland 21228. |
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