A rapid and sensitive assay for arginase |
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| Authors: | Urs T. Rüegg Anthony S. Russell |
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| Affiliation: | 1. Department of Biochemistry, Faculty of Science, University of Geneva, 1211 Geneva 4, Switzerland;2. Rheumatic Diseases Unit, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada |
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| Abstract: | An assay for arginase is described that uses l-[guanido-14C]arginine as substrate. Unhydrolyzed arginine is removed in a batch procedure with sulfonate resin and the [14C]urea product is determined quantitatively in the resin supernatant. The assay requires 5 min and is performed in one tube. The sensitivity is approximately 0.1 munits of arginase. Arginase activities in fetal calf serum and in murine macrophage extract have been determined and the bovine liver enzyme has been used as a reference. |
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| Keywords: | To whom reprint requests should be sent. |
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