Iodotyrosylation of peptides using tertiary-butyloxycarbonyl-l-[125I]iodotyrosine N-hydroxysuccinimide ester |
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Authors: | Richard K Assoian Petra M Blix Arthur H Rubenstein Howard S Tager |
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Institution: | 1. Department of Biochemistry The University of Chicago, Chicago, Illinois 60637 USA;2. Department of Medicine, The University of Chicago, Chicago, Illinois 60637 USA |
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Abstract: | A rapid method for the iodotyrosylation of peptides using tertiary-butyloxycarbonyl-l-125I]-iodotyrosine N-hydroxysuccinimide ester is described. The method, like that of Bolton and Hunter (1973, Biochem. J.133, 529–539), uses nonoxidizing conditions, is applicable to tyrosine-free peptides, and results in an easily isolated product with decreased cationic charge. The present method has the additional advantage that the derivatized peptide is readily deblocked to form a radiolabeled product with the same net charge as the starting material. |
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