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Reconstruction of cell population dynamics using CFSE |
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| Authors: | Andrew Yates Cliburn Chan Jessica Strid Simon Moon Robin Callard Andrew JT George Jaroslav Stark |
| Institution: | (1) Department of Biology, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA;(2) Department of Biostatistics and Bioinformatics, Duke University Laboratory of Computational Immunology, 106 North Bldg, Research Drive, Box 90090, Durham, NC 27708, USA;(3) Peter Gorer Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, King's College London, Guy's Hospital, London, SE1 9RT, UK;(4) Department of Mathematics, Imperial College London, 180 Queen's Gate, London, SW7 2BZ, UK;(5) Centre for Integrative Systems Biology at Imperial College (CISBIC), London, UK;(6) Immunobiology Unit, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK;(7) Department of Immunology, Faculty of Medicine, Imperial College London, Hammersmith Hospital, London, W12 0NN, UK |
| Abstract: | BackgroundQuantifying cell division and death is central to many studies in the biological sciences. The fluorescent dye CFSE allows the tracking of cell division in vitro and in vivo and provides a rich source of information with which to test models of cell kinetics. Cell division and death have a stochastic component at the single-cell level, and the probabilities of these occurring in any given time interval may also undergo systematic variation at a population level. This gives rise to heterogeneity in proliferating cell populations. Branching processes provide a natural means of describing this behaviour. |
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