Scarless and site-directed mutagenesis in Salmonella enteritidischromosome |
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| Authors: | Mandy M Cox Sherryll L Layton Tieshan Jiang Kim Cole Billy M Hargis Luc R Berghman Walter G Bottje Young Min Kwon |
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| Affiliation: | (1) Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA;(2) Department of Poultry Science and Veterinary Pathobiology, Texas A&M University, College Station, TX, USA |
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| Abstract: | Background A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for vaccine development. Most methods use a multi-step approach that requires cloning and/or designing repeat sequences to facilitate homologous recombination. We have modified previously published techniques to develop a simple, efficient PCR-based method for scarless insertion of DNA into Salmonella enteritidis chromosome. |
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