|
|||||
|
|
Phenylalanine and tyrosine ammonia-lyase activity in Sporobolomyces pararoseus |
|
| Authors: | J R Parkhurst and D S Hodgins |
| Institution: | Department of Biochemistry and Molecular Biology, University of Oklahoma School of Medicine, and The Oklahoma Medical Research Foundation, 800 NE 13th Street, Oklahoma City, Oklahoma 73104, U.S.A. |
| Abstract: | Phenylalanine ammonia-lyase from Sporobolomyces pararoseus was purified more than 450-fold. Polyacrylamide disc gel electrophoresis of this purified enzyme gave a single major protein band. Tyrosine ammonia-lyase activity was monitored during the purification of phenylalanine ammonia-lyase. Deaminating activities for phenylalanine and tyrosine were not separated during the purification process. The existence of one ammonia-lyase with bisubstrate activity is postulated. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |