Purification and Some Properties of Cytochrome b-560 from Nitrosomonas europaea |
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Authors: | Fukumori, Yoshihiro Saito, Takashi Yamazaki, Takeshi Yamanaka, Tateo |
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Affiliation: | Department of Life Science, Faculty of Science, Tokyo Institute of Technology O-okayama, Meguro-ku, Tokyo 152, Japan |
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Abstract: | Cytochrome b-560 was purified to an electrophoretically homogeneousstate from Nitrosomonas europaea. It showed absorption peaksat 427, 530 and 560 nm in the reduced form. Its molecular weightwas estimated to be 44,000 by SDS-polyacrylamide gel electrophoresisand the same value was obtained on the basis of the contentsof haem and protein. The cytochrome was not autoxidizable anddid not react with CO. 1Present address: Tokyo Research Center, TOSOH Corporation,Hayakawa, Ayase-shi, Kanagawa 252, Japan2Present address: Faculty of Integrated Arts and Sciences, HiroshimaUniversity, Higashisenda-machi, Hiroshima 730, Japan (Received March 23, 1988; Accepted June 2, 1988) |
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