Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death |
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| Authors: | Youn Wook Chung Daewon Jeong Ok Jeong Noh Yong Hwan Park Soo Im Kang Min Goo Lee Tae-Hoon Lee Moon Bin Yim Ick Young Kim |
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| Institution: | 1.Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology,Korea University,Seoul,Korea;2.Laboratory of Biochemistry, National Heart, Lung, and Blood Institute,National Institutes of Health,Bethesda,USA;3.Department of Microbiology and Aging-associated Disease Research Center,Yeungnam University College of Medicine,Daegu,Korea;4.School of Dentistry, Dental Science Research Institute, The 2nd Stage of Brain Korea 21 for the Dental School,Chonnam National University,Gwangju,Korea |
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| Abstract: | It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats,
and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased
in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1
and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with
small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced
by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken
together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal
development. |
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| Keywords: | antioxidant neuronal cells oxidative stress selenium selenoprotein W |
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