四溴甘脲对枯草杆菌黑色变种芽胞损伤作用的电镜观察

为探索四溴甘脲消毒剂杀灭细菌的机理,采用透射电镜技术对四溴甘脲消毒剂处理过的枯草杆菌黑色变种芽胞的超微结构进行了分析和比较.结果显示,以含有效溴274mg/L的四溴甘脲消毒剂作用30min,可使枯草杆菌黑色变种芽胞杀灭率达到100%.在透射电镜下观察到,经该消毒剂作用的枯草杆菌黑色变种芽胞壳质破损断裂明显,壳内结构模糊,核心溶解,有的芽胞近似空壳.结果显示,四溴甘脲消毒剂杀灭芽胞效果优于普通含氯消毒剂,对细菌芽胞超微结构破坏明显.

Electronmicroscope observation on injurious effect of tetrabromo-glycoluril on Bacillus subtilis var. niger spore

To explore the mechanism of action of tetrabromo-glycoluril disinfectant in killing bacteria,the transmission electronmicroscope technique was used to analyze and compare the ultrasturcture of spores of Bacillus subtilis var.niger treated with tetrabromo-glycoluril disinfectant.The results showed that the killing rate of spores of Bacillus subtilis vat.niger exposed to the solution of tetrabromo-glycohril disinfectant containing available bromine 274mg/L for 30min was 100%.Transmission electronmicroscopic examination showed that the coat of spores of B.subtilis vat.after exposure to the disinfectant damaged obviously.The structure of coat inclusion became indistinct and the core of nucleus lysed.Some spores became nearly hollow.The results indicated that the sporicidal efficacy of tetrabromo-glycoluril disinfectant was better than that of ordinary chlorine-containing disinfectants and could destroy obviously the ultrasturcture of bacterial spores.