| 中华眼镜蛇毒心脏毒素对人肝癌细胞株细胞膜的影响 |
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| 引用本文： | 龚海云 乐毅. 中华眼镜蛇毒心脏毒素对人肝癌细胞株细胞膜的影响[J]. 中国应用生理学杂志, 1999, 15(4): 354-357 |
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| 作者姓名： | 龚海云 乐毅 |
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| 作者单位： | 广州医学院广州蛇毒研究所!广州510182(龚海云，余清声，管锦霞)，第一军医大学生化教研室!广州510515(乐毅) |
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| 摘 要： | 目的和方法：运用５７０ 型粘附式细胞仪光漂白后荧光再分布法测定单个细胞膜脂流动性的动态变化和流式细胞仪测定细胞群细胞膜电位，以观察中华眼镜蛇毒心脏毒素对人肝癌细胞株细胞膜的影响。结果：心脏毒素使肝癌细胞膜脂流动性下降，并且使肝癌细胞膜电位下降。结论：心脏毒素对人肝癌细胞株Ｈ７４０２细胞膜有损伤作用
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| 关 键 词： | 眼镜蛇毒 心脏毒素 膜脂流动性 细胞膜电位 人肝癌细胞株 |
EFFECT OF CARDIOTOXIN FROM NAJA NAJA ATRA VENOM ON CELL MEMBRANE OF HUMAN LIVER CANCER CELL LINE |
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| Gong Haiyun,Le Yi,Yu Qingsheng,Guan Jinxia. EFFECT OF CARDIOTOXIN FROM NAJA NAJA ATRA VENOM ON CELL MEMBRANE OF HUMAN LIVER CANCER CELL LINE[J]. Chinese journal of applied physiology, 1999, 15(4): 354-357 |
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| Authors: | Gong Haiyun Le Yi Yu Qingsheng Guan Jinxia |
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| Affiliation: | Gong Haiyun,1Le Yi,Yu Qingsheng,Guan Jinxia |
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| Abstract: | Aim and Methods: To observe the effect of cardiotoxin from Naja naja atra on cell membrane of human liver cancer cell line, membrane lipid fluidity of single cells was measured by the technique of fluorescence redistribution after photobleaching by using Adherent Cell Analysis and Sorting Cytometer type 570. Membrane potential of cell population was measured by flow cytometer (type EPICS ELITE). Results: Cardiotoxin reduced membrane lipid fluidity and membrane potential of human liver cancer cells. Conclusion: Cardiotoxin can damage cell membrane of human liver cancer cell line H 7402 . |
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| Keywords: | Cobra snake venom Cardiotoxin Membrane lipid fluidity Membrane potential Human liver cancer cell line |
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