慢性呼吸衰竭病人红细胞膜蛋白和红细胞变形能力的研究

目的：探讨红细胞膜蛋白在红细胞变形性改变中的作用。方法：参照Ｌｅａｍｍｌｉ和Ｐｅａｃｏｃｋ方法，测定了肺心病Ⅰ型呼吸衰竭（Ⅰ组）１８例、Ⅱ型呼吸衰竭（Ⅱ组）１８例和健康对照（ＣＧ）２０例的红细胞膜带３蛋白、膜收缩蛋白二聚体（ＳｐＤ）和四聚体（ＳｐＴ）的相对含量与红细胞变形能力。结果：Ⅰ、Ⅱ组带３蛋白、ＳｐＤ、ＳｐＴ相对含量和红细胞变形指数（ＤＩ）与对照组均有显著差异，且肺心病病人的ＤＩ与带３蛋白相对含量呈显著正相关，与ＳｐＤ／ＳｐＴ比值呈显著负相关。结论：带３蛋白和膜收缩蛋白的异常，可能是导致肺心病人红细胞变形能力降低的重要因素之一。

STUDY OF ERYTHROCYTE MEMBRANE PROTEINS AND DEFORMABILITY IN PATIENTS WITH RESPIRATORY FAILURE

Aim and Methods:To investigate the role of erythrocyte membrane protein in the changes of erythrocyte deformability. The changes of erythrocyte deformability and band 3 protein and spectrin dimers(Sp D) and tetramers (Sp T) were studied in 18 cases of respiratory fairlure type I (group I) and 18 cases of respiratory failure type II (group II) and 15 normal subjects,using the methods of Chang,Liammli and peacock. Results: The erythrocyte deformation index and relative content of band 3 protein and Sp T were significantly lower in group I and II than that in CG . The relative content of Sp D and ratio of Sp D/Sp T were signficanitly higher in group I and II than in CG. The erythrocyte deformability in group I and II were positively related to the ratio of Sp D/Sp T. Conclusion: These findings suggested that the abnormality of band 3 and spectrin may be one of the reasons that resulted in erythrocyte deformability decrease in cor pulmonale patients with respiratory failure.