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| 不同种型布鲁菌间的基因获得与缺失研究 | |
| 引用本文： | 杨毅,颜焱峰,陈燕芬,周晓燕,王玉飞,周冬生,杨瑞馥,姜海,崔步云,陈泽良,黄留玉. 不同种型布鲁菌间的基因获得与缺失研究[J]. 生物技术通讯, 2011, 22(3): 358-361. DOI: 10.3969/j.issn.1009-0002.2011.03.012 |
| 作者姓名： | 杨毅 颜焱峰 陈燕芬 周晓燕 王玉飞 周冬生 杨瑞馥 姜海 崔步云 陈泽良 黄留玉 |
| 作者单位： | 1. 军事医学科学院疾病预防控制所,北京,100071 2. 军事医学科学院微生物流行病研究所,北京,100071 3. 中国疾病预防控制中心,传染病预防控制所,北京,102206 |
| 基金项目： | 国家重点基础研究发展计划，国家传染病重大专项，国家自然科学基金 |
| 摘 要： | 目的:了解不同种型布鲁菌间的基因差异及基因的获得与缺失情况。方法:采用生物信息学方法比较分析已测序的10株布鲁菌基因水平的差异,分析它们的核心基因组与泛基因组,对得到的差异基因用PCR验证其在19株不同生物型标准菌株中的分布情况。结果:不同种型布鲁菌在基因水平上存在较大差异,差异基因主要位于Ⅱ号染色体上;根据差异基因,鉴定了42个差异区段,这些差异区段在19株不同生物型标准菌株中存在差异分布。结论:布鲁菌在进化过程中分别获得或失去了不同的基因区段,从而适应不同的宿主环境。 |
| 关 键 词： | 布鲁菌 比较基因组学 差异区段 基因获得与缺失 |
Gene Lost and Found Among Different Brucella Species and Bio- types |
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| YANG Yi,YAN Yan-Feng,CHEN Yan-Fen,ZHOU Xiao-Yan,WANG Yu-Fei,ZHOU Dong-Sheng,YANG Rui-Fu,JIANG Hai,CUI Bu-Yun,CHEN Ze-Liang,HUANG Liu-Yu. Gene Lost and Found Among Different Brucella Species and Bio- types[J]. Letters in Biotechnology, 2011, 22(3): 358-361. DOI: 10.3969/j.issn.1009-0002.2011.03.012 | |
| Authors: | YANG Yi YAN Yan-Feng CHEN Yan-Fen ZHOU Xiao-Yan WANG Yu-Fei ZHOU Dong-Sheng YANG Rui-Fu JIANG Hai CUI Bu-Yun CHEN Ze-Liang HUANG Liu-Yu |
| Affiliation: | 1a 1.a.Institute of Disease Control and Prevention;b.State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology;Academy of Military Medical Sciences,Beijing 100071;2.National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206;China |
| Abstract: | Objective：To probe into the genetic differences and gene lost and found as well among different Brucella species and biotypes.Methods：The whole genome sequence of the 10 sequenced Brucella strains were compared,and the core-genome and pan-genome of Brucella were putatively analyzed.The different genes were analyzed by PCR to identify their distribution among the 19 Brucella biotypes.Results：Great differences in gene composition were found among these strains,and these differences were mainly located on chromosome Ⅱ.Of these differently distributed genes,42 different regions were identified and distributed among the 19 biotypes.Conclusion：The results implied that during Brucella evolution,gene found and lost have been occurred for Brucella adaptation to various host environments. |
| Keywords: | Brucella comparative genomics different region gene found and lost |
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