|
|||||
|
|
| 慢病毒介导RNA干扰HPIP蛋白抑制肿瘤细胞增殖 | |
| 引用本文： | 徐小洁,范忠义,韩永健,张浩,丁丽华,韩聚强,王凌雪,杨智洪,杜楠,叶棋浓.慢病毒介导RNA干扰HPIP蛋白抑制肿瘤细胞增殖[J].生物技术通讯,2011,22(3):321-324. |
| 作者姓名： | 徐小洁 范忠义 韩永健 张浩 丁丽华 韩聚强 王凌雪 杨智洪 杜楠 叶棋浓 |
| 作者单位： | 1. 军事医学科学院,生物工程研究所,北京,100850 2. 解放军总医院,第一附属医院,北京,100048 3. 北京军区总医院,北京,100700 |
| 基金项目： | 国家自然科学基金，国家重大科学研究计划，全军医药卫生科研基金 |
| 摘 要： | 目的:建立高效稳定的造血相关的PBX相互作用蛋白质(HPIP)小干扰RNA(siRNA)细胞导入方法,检测HPIP的表达对肿瘤细胞生长增殖的影响。方法:构建人HPIP慢病毒siRNA干扰载体,将重组质粒转染人胚肾293T细胞,通过实时定量RT-PCR及Western印迹分析检测Lenti-H1 HPIP siRNA的干扰效果;将Lenti-H1 HPIPsiRNA与4个包装质粒共同转染293T细胞,包装成慢病毒后,感染宫颈癌HeLa和肝癌HepG2细胞,经嘌呤霉素筛选2周后,收集细胞进行Western印迹检测;用结晶紫实验检测其对肿瘤细胞生长增殖的影响。结果:构建的Lenti-H1 HPIP siRNA能有效抑制HPIP的表达;结晶紫实验显示,慢病毒介导的HPIP siRNA导致细胞增殖的显著抑制。结论:慢病毒介导的HPIP敲减能明显抑制肿瘤细胞的增殖,HPIP可能是一个潜在的肿瘤治疗新靶点。 |
| 关 键 词： | 造血相关的PBX相互作用蛋白质 RNA干扰 慢病毒 细胞增殖 |
Lentiviral-Mediated Knock-Down of Human HPIP Inhibits Cell Growth and Proliferation |
|
| XU Xiao-Jie,FAN Zhong-Yi,HAN Yong-Jian,ZHANG Hao,DING Li-Hua,HAN Ju-Qiang,WANG Ling-Xue,YANG Zhi-hong,DU Nan,YE Qi-Nong.Lentiviral-Mediated Knock-Down of Human HPIP Inhibits Cell Growth and Proliferation[J].Letters in Biotechnology,2011,22(3):321-324. | |
| Authors: | XU Xiao-Jie FAN Zhong-Yi HAN Yong-Jian ZHANG Hao DING Li-Hua HAN Ju-Qiang WANG Ling-Xue YANG Zhi-hong DU Nan YE Qi-Nong |
| Institution: | XU Xiao-Jie1,FAN Zhong-Yi2,HAN Yong-Jian1,ZHANG Hao1,DING Li-Hua1,HAN Ju-Qiang3,WANG Ling-Xue1,YANG Zhi-hong1,DU Nan2,YE Qi-Nong1 1.Beijing Institute of Biotechnology,Beijing 100850,2.First Affiliated Hospital of China PLA General Hospital,Beijing 100048,3.Beijing Military General Hospital,Beijing 100700,China |
| Abstract: | Objective:To establish an efficient and stable way to deliver hematopoietic PBX-interacting protein(HPIP) small interfering RNA(siRNA) and examine the effect of HPIP siRNA on cell proliferation.Methods:pLenti-H1/HPIP siRNA was constructed and transfected into 293T cells.Western blot and real-time RT-PCR were carried out to detect the inhibitory effect of Lenti-H1 HPIP siRNA.The four viral packaging vectors together with pLenti-H1/HPIP siRNA were used to transfect 293T cells to produce lentivirus particles.A... |
| Keywords: | hematopoietic PBX-interacting protein lentivirus small interfering RNA cell proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |