| 红树植物叶片总RNA提取方法比较与优化 |
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| 引用本文： | 宋晖,王友绍.红树植物叶片总RNA提取方法比较与优化[J].生态科学,2011,30(2):201-206. |
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| 作者姓名： | 宋晖 王友绍 |
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| 作者单位： | 1. 中国科学院南海海洋研究所热带海洋环境动力学重点实验室, 广州, 510301; 2. 中国科学院大亚湾海洋生物综合试验站, 深圳, 518121; 3. 中国科学院研究生院, 北京, 100039 |
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| 基金项目： | "十一五"国家科技支撑计划重点项目，国家自然科学基金项目，中国科学院知识创新工程重要方向性项目 |
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| 摘 要： | 分别采用三种植物RNA提取试剂盒、改良的CTAB-LiCl法和CTAB-异丙醇法五种方法提取四种红树植物叶片总RNA,并用紫外光谱、琼脂糖凝胶电泳、cDNA合成和real-time PCR等手段对提取效果进行鉴定.反复试验表明:五种方法对白骨壤Aricennia marina叶片总RNA提取均有良好的效果.Tiangen RNA提取试剂能够成功提取秋茄Kandelia candel、桐花树Aegiceras corniculatum、白骨壤的总RNA,提取的木榄Bruguiera gymnorrhiza总RNA浓度低、杂质较多,且稳定性较差,在对该方法改良后则能够成功提取木榄的总RNA.Invitrogen试剂盒对木榄和秋茄叶片均有良好的提取效果,但是对桐花树提取的RNA易受到蛋白的污染.CTAB-LiCl法和CTAB-异丙醇法提取的RNA总产量偏低,提取时间较长,且实时定量结果表明,两种方法反转录效率较试剂盒方法也偏低.
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| 关 键 词： | 红树植物 总RNA 提取 方法比较 |
| 收稿时间： | 2011-04-25 |
Analysis and improvement of high-quality RNA extraction in leaves of mangrove plants |
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| SONG Hui,WANG You-shao.Analysis and improvement of high-quality RNA extraction in leaves of mangrove plants[J].Ecologic Science,2011,30(2):201-206. |
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| Authors: | SONG Hui WANG You-shao |
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| Institution: | SONG Hui~(1,2,3),WANG You-shao~(1,2*) 1.Key Laboratory of Tropical Marine Environmental Dynamics,South China Sea Institute of Oceanology,ChineseAcademy of Sciences,Guangzhou 510301,China 2 Marine Biology Research Station at DayaBay,Chinese Academy of Sciences,Shenzhen 518121,China 3.GraduateSchool of the ChineseAcademy of Sciences,Beijing 100039,China |
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| Abstract: | Five extraction methods including Takara RNA,Tiangen kit,Invitrogen kit,modified CTAB-LiCl and CTAB-isopropyl alcohol method were compared for extracting total RNA from the leaves of four mangrove plants(Bruguiera gymnorrhiza,Kandelia candel,Aegiceras corniculatum,and Aricennia marina).The five methods were all suitable for total RNA extraction in leaves of A. marina.Tiangen kit was successfully used to extract the RNA in leaves of A.corniculatum,A.marina,and K.candel,but failed to extract the RNA in leaves... |
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| Keywords: | Mangrove plants total RNA extraction analysis |
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