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| 脂肪干细胞的共培养及其分化应用概述 | |
| 引用本文： | 聂绪强,陈怀红,唐宁,卞卡. 脂肪干细胞的共培养及其分化应用概述[J]. 生物工程学报, 2011, 27(8): 1121-1131 |
| 作者姓名： | 聂绪强 陈怀红 唐宁 卞卡 |
| 作者单位： | 1. 上海中医药大学穆拉德中药现代化研究中心,上海,201203 2. 浙江大学医学院附属二院,杭州,310009 3. 上海中医药大学穆拉德中药现代化研究中心,上海201203;上海市教委一氧化氮和炎症医学E-研究院,上海201203;美国德克萨斯大学休斯敦医学院综合生物及药理学系德克萨斯大学分子医学研究所,休斯敦TX77030 |
| 基金项目： | 国“十一五”国家科技支撑计划 (No. 2006 BAIⅡB08-03)，上海市教委高校一氧化氮与炎症医学E研究院计划 (No. E-04010)，上海市科委中药现代化专项 (No. 08DZ1972104)，国家科技部重大专项重大新药创制项目 (No. 2009ZX09311-003) 资助。 |
| 摘 要： | 干细胞在体外特定培养条件下可以被诱导分化成具有不同体细胞表型的细胞。除了通过不同培养条件进行体外诱导分化的方法外，用成熟体细胞与干细胞共培养同样可以诱导干细胞定向分化。以下首先简述了脂肪干细胞 (Adipose-derived stem cells，ADSCs) 的来源及其标志，然后重点就ADSCs的不同培养方法、诱导分化及最新的临床应用进行阐述，包括药物及化学诱导培养、体细胞与ADSCs二维、三维共培养等，最后提出ADSCs的问题所在并对此技术进行展望。 |
| 关 键 词： | 脂肪干细胞，共培养，流式细胞仪，组织工程，再生医学 |
| 收稿时间： | 2010-12-07 |
Progress in co-culture and differentiation in adipose-derived stem cells: a review |
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| Xuqiang Nie,Huaihong Cheng,Ning Tang and Ka Bian. Progress in co-culture and differentiation in adipose-derived stem cells: a review[J]. Chinese journal of biotechnology, 2011, 27(8): 1121-1131 | |
| Authors: | Xuqiang Nie Huaihong Cheng Ning Tang Ka Bian |
| Affiliation: | Murad Research Center for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The 2nd Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China;Murad Research Center for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Center for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; E-Institute of Shanghai Universities, Division of Nitric Oxide and Inflammatory Medicine, Shanghai 201203, China; Department of Integrative Biology and Pharmacology, Institute of Molecular Medicine, University of Texas Medical School, Houston TX77030, USA |
| Abstract: | Stem cells can be differentiated into many kinds of somatic cells under defined culture conditions. In addition, the homing possess can be partially imitated by co-culture of stem cells with mature somatic cells. Regarding the importance of clinical application of adipose-derived stem cells (ADSCs), our review first introduced the sources and signs of ADSCs, and then the current knowledge of ADSCs co-culture technology, including drug and chemical induced culture, two-dimensional (2D) and three-dimensional (3D) co-culture, mechanisms of ADSCs differentiation, and application development in recent years in details. Finally, we also addressed prospects of ADSCs. |
| Keywords: | adipose-derived stem cells (ADSCs) co-culture flow cytometry tissue engineering regenerative medicine |
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