Interleukin-15 interactions with interleukin-15 receptor complexes: characterization and species specificity |
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Authors: | Eisenman June Ahdieh Minoo Beers Courtney Brasel Kenneth Kennedy Mary K Le Tiep Bonnert Timothy P Paxton Raymond J Park Linda S |
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Institution: | Department of Protein Chemistry, Immunex Corporation, 51 University Street, Seattle, WA, 98101, USA. eisenman@immunex.com |
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Abstract: | Interleukin (IL-) 2 and IL-15 share the IL-2 receptor betagamma c subunits (IL-2Rbetagamma c) but have specific, unique alpha receptor subunits. We studied species specificity of human (hu), simian (si), and mouse (mu) IL-15 and found that hu and si IL-15 behaved similarly in all systems investigated. Hu and mu IL-15 bound hu or mu IL-15Ralpha with equal high affinity in the presence or absence of IL-2Rbetagamma c and exhibited similar proliferative activities on cells containing all three subunits. However, quantitative differences were noted in the specific activity of hu and mu IL-15 in both in vitro and in vivo systems utilizing IL-2Rbetagamma c in the absence of IL-15Ralpha. These data show that hu IL-15 may be used in mouse model systems, however care must be taken when comparing the efficacy and toxicity of cytokines across species. |
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