Hexokinase activity as marker to assess time of harvest of foot-and-mouth disease virus in BHK21 Cl13 cell cultures |
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Authors: | Suryanarayana V V Banumathi N Rao B U |
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Institution: | Indian Veterinary Research Institute, Bangalore-560024, (INDIA). |
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Abstract: | Hexokinase (B.C. 2.7.1.1) activity as a marker enzyme during FMD viral infection has been observed spectrophotometrically in a system coupled with glucose-6-phosphate dehydrogenase, in supernatants of BHK(21)Cl(13) suspension as well as anchored cell culture at a minimum of 10(4) infective virus particles/ml. Specific activity increased with virus concentration in culture supernatants and abruptly decreased with a fall in virus titer, as has been noted by TCID/50,146 S concentration, and enzyme-linked immunosorbent assay (ELISA) readings. |
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