A simple method to preserve algal spores of <Emphasis Type="Italic">Ulva</Emphasis> spp. in cold storage with ampicillin |
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Authors: | Hari Datta Bhattarai Babita Paudel Yong-Ki Hong Hyun Woung Shin |
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Institution: | (1) Department of Marine Biotechnology, Soonchunhyang University, Asan, 336-745, South Korea;(2) Department of Biotechnology, Pukyong National University, Namku, Busan, 608-737, Korea |
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Abstract: | Preservation of algal spores of the green seaweed Ulva fasciata and U. pertusa was enhanced by the addition of ampicillin in f/2 medium at 4°C. The viability of preserved spores was determined by a spore
germination assay at various time intervals. The germination rate of U. fasciata remained at 5% to 38% for the first five days, dropping to 1% to 6% on the 10th day of storage with various preservation
treatments without ampicillin at 4°C during parameter-selecting experiments. In f/2 medium, 53% of U. fasciata spores were still viable on day 5 and 23% on day 10 at 4°C. By adding 100 μg mL−1 ampicillin to f/2 medium, 90% of the spores were viable at day 40 and 61% after 100 days of storage at 4°C. Spores of U. pertusa had lower preservation rates, with viabilities of 70% at day 40 and 32% at day 100. Algal spore preservation was heavily
dependent on the bacterial contamination and subsequent degradation in stock solutions.
Handling editor: L. Naselli-Flores |
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Keywords: | Cold shock Cryo-injury Spore preservation Ulva fasciata Ulva pertusa |
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