马鹿卵母细胞体外培养与体外受精的超微结构

用FSH 对马鹿进行超数排卵,通过抽吸法和切割法采集卵泡卵母细胞,用M199 为基础的培养液在38.5℃、5%CO₂ 和饱和湿度条件下对马鹿卵母细胞进行体外培养与体外受精培养,利用透射电镜观察不同时期马鹿卵母细胞的超微结构,旨在揭示马鹿卵母细胞体外培养前、培养后及受精后超微结构的变化规律。结果表明,培养前卵丘细胞紧紧包围卵母细胞,卵母细胞表面的微绒毛细长,伸入透明带内,皮质区及细胞中心分布大量的细胞器。培养后卵丘细胞与卵母细胞结合松散,卵母细胞表面微绒毛短粗,倒伏于卵表面,第一极体无核,皮质颗粒在皮质区成层排列,细胞质中细胞器分布均匀。受精后卵母细胞表面的微绒毛由倒伏而竖起,第二极体有核,细胞质中细胞器丰富,主要分布于细胞中心。

The ultrastructure of wapiti (Cervus elaphus) oocytes in vitro cultivation and fertilization

The aim was to study the role of ultrastructural changes of wapiti oocytes before and after cultivation and fertilization in vitro. The oocytes were collected by aspiration and deep cut methods from deer that had been injected with FSH, for superovulation. The oocytes were selected from different developmental stages and their ultrastructure was observed under the Transmission Electron Microscope. Cultivation and fertilization in M199 was carried out at 38.5℃ under a humidified atmosphere of 5% CO₂ in the air. The results revealed that before the oocytes were cultivated in vitro,the cumulus cells surrounded them tightly;long thin microvilli were on their surface and deep into the zona pellucida. There were a lot of organelles in the area of the cortex and the centre of the cells. After the oocytes were cultivated in vitro,the link between cumulus cells and oocytes became loose,the microvilli lying on the oocytes surface shortened and thickened,the first polar body had no nucleus,the cortical granules were distributed in layers in the cortex area,and the organelles were distributed evenly in the cytoplasm. When the oocytes were fertilized in vitro,the microvilli on their surface became erect, the second polar body had a nucleus,and in the cytoplasm of the oocytes there were abundant organelles which were mainly distributed in the centre of the cells.