A multi-spectroscopic and molecular docking approach to investigate the interaction of antiviral drug oseltamivir with ct-DNA |
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| Authors: | Neda Hosseinpour Moghadam Nahid Shahabadi Reza Golbedaghi |
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| Institution: | 1. Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran;2. Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran;3. Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran;4. Department of Chemistry, Payame Noor University, Hamedan, Iran |
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| Abstract: | The possible interaction between the antiviral drug oseltamivir and calf thymus DNA at physiological pH was studied by spectrophotometry, competitive spectrofluorimetry, differential pulse voltammogram (DPV), circular dichroism spectroscopy (CD), viscosity measurements, salt effect, and computational studies. Intercalation of oseltamivir between the base pairs of DNA was shown by a sharp increase in specific viscosity of DNA and a decrease of the peak current and a positive shift in differential pulse voltammogram. Competitive fluorescence experiments were performed using neutral red (NR) as a probe for the intercalation binding mode. The studies showed that oseltamivir is able to release the NR. |
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| Keywords: | Oseltamivir DNA interaction molecular docking DFT DPV intercalation |
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