Evaluation of fractionatedWuchereria bancrofti microfilarial excretory-secretory antigens for diagnosis of bancroftian filariasis by enzyme linked immunosorbent assay |
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| Authors: | M V R Reddy Ashok Malhotra G B K S Prasad B C Harinath |
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| Institution: | (1) Department of Biochemistry, M. G. Institute of Medical Sciences, Sevagram, 442 102 Wardha, India |
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| Abstract: | TheWuchereria bancrofti microfilarial excretory-secretory antigens were fractionated into ES1, ES2, ES3 and ES4 by ultra-membrane filtration and
evaluated for their diagnostic utility by enzyme linked immunosorbent assay. Three of the four fractions showed antigenic
activity (ES2, ES3 and ES4). The antigen fractions ES2 and ES4 were highly active in the detection of filarial IgM antibody
in clinical filariasis and microfilaraemia respectively. The chemical characterization of the ES2 and ES4 antigen fractions
showed that they were glycoproteins |
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| Keywords: | Microfilarial excretory-secretory antigen ultramembrane filtration enzyme linked immunosorbent assay |
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