Physicochemical,catalytic, and regulatory properties of malate dehydrogenase from Rhodovulum steppense bacteria,strain A-20s |
| |
| Authors: | A. T. Eprintsev M. I. Falaleeva I. V. Parfenova M. S. Lyashchenko E. I. Kompantseva A. Yu. Tret’yakova |
| |
| Affiliation: | 1. Voronezh State University, Universitetskaya pl. 1, Voronezh, 394006, Russia
2. Vinogradskii Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7/2, Moscow, 117811, Russia
|
| |
| Abstract: | The physicochemical, regulatory, and kinetic properties of malate dehydrogenase (EC 1.1.1.37) from haloalkaliphilic purple nonsulfur Rhodovulum steppense bacteria, strain A-20s, were studied. The malate dehydrogenase (MDH) preparation with a specific activity of 3.775 ± 0.113 U/mg protein was obtained in an electrophoretically homogeneous state using multistep purification. Using homogenous preparations, the molecular weight and the Michaelis constant of the enzyme were determined; the effects of metal ions, the temperature effect, and the thermal stability of the MDH were studied. The dimer structure of the enzyme was demonstrated by DS-Na-electrophoresis. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
