家蚕小热激蛋白家族成员Hsp23.7基因的克隆与分析

Hsp23.7基因是小热激蛋白家族的成员,本文研究了家蚕BombyxmoriL.的Hsp23.7基因,并对其进行了原核表达,获得了相应分子量的表达产物。推导的开放阅读框编码210个氨基酸,分子量为23.7ku,等电点为5.17。同时,利用实时定量PCR技术对Hsp23.7基因在家蚕不同组织的表达谱进行了鉴定。结果显示Hsp23.7基因在5龄幼虫时期的各组织中都有表达,在卵巢中表达量最高,达到3.64×107拷贝数/μg,其次在脂肪体,翅原基,马氏管中表达量也较高,在血淋巴中表达量最低,仅为7.11×103拷贝数/μg。

Cloning and analysis of small heat-shock gene Hsp23.7 from Bombyx mori

Hsp23.7 is one of the small heat-shock family of proteins.The Hsp23.7 gene of Bombyx mori L.（Bm-Hsp23.7） was cloned and expressed in E.coli.The candidate ORF of Bm-Hsp23.7 contains 210 amino acids.Its molecular weight（mw） is 23.7 ku and isoelectric point（pI） is 5.17.Real-time PCR data was used to reveal the profile of the spatio-temporal expression pattern of Bm-Hsp23.7.Absolute quantization demonstrated that Bm-Hsp23.7 was expressed at different levels in all tissues of 5th instar larva of B.mori.The highest level of expression was in the ovary（3.64 × 107 copy numbers /μg）,followed by the fat bodies,then the wing discs and malpighian tubules.The lowest level of expression was in hemocytes（7.11 × 103 copy numbers /μg）.