DNA barcoding of genus Metapenaeopsis (Decapoda: Penaeidae) and molecular phylogeny inferred from mitochondrial and nuclear DNA sequences |
| |
| Institution: | 1. AUT Lab for Cephalopod Ecology & Systematics, School of Science, Auckland University of Technology, Auckland, New Zealand;2. Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland 4810, Australia;3. Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR, USA;4. National Museum of Nature and Science, Curator Emeritus, 4-1-1 Amakubo, Tsukuba, Ibaraki 305-0005, Japan;5. CEFAS, Pakefield Rd., Lowestoft NR330HT, United Kingdom;6. Instituto de Investigaciones Marinas de Vigo (IIM-CSIC), Vigo, Spain;1. Institut de Ciencies del Mar (CSIC), Passeig maritim de la Barceloneta 37-49, CP 08003 Barcelona, Spain;2. Centre d’estudis avançats de Blanes (CSIC), C/ d’accés a la Cala St.Francesc 14, 17300 Blanes, Spain;1. Biology Department, Seattle University, Seattle, WA 98122, USA;2. National Museum of Natural History, Smithsonian Institution, Washington, DC 20013, USA;3. Department of Biology, University of Miami, Coral Gables, FL 33143, USA;4. Tropical Marine Science Institute, National University of Singapore, Singapore 119227, Republic of Singapore;5. Department of Biology, Tennessee Tech University, Cookeville, Tennessee, 38505, USA;1. Laboratório de Biologia de Camarões Marinhos e Dulcícolas (LABCAM), Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista (UNESP), Av. Eng. Luiz Edmundo Corrijo Coube, 14-01, 17033-360 Bauru, SP, Brazil;2. Laboratório de Bioecologia e Sistemática de Crustáceos (LBSC), Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo (USP), 14040- 901, Ribeirão Preto, São Paulo, Brazil |
| |
| Abstract: | Metapenaeopsis Bouvier, 1905 is the most diverse genus within Penaeidae. Metapenaeopsis shrimps exhibited subtle morphological differences, which make identification a difficult task based on taxonomic keys alone. In this study, we carried out DNA barcoding and phylogenetic analyses to examine taxonomy and phylogeny of genus Metapenaeopsis based on mitochondrial (COI) and nuclear (PEPCK and NaK) genes. Despite limited performance of DNA barcoding in delineating Metapenaeopsis shrimps, it questioned the taxonomic status of the two subspecies, Metapenaeopsis mogiensis intermedia and Metapenaeopsis mogiensis mogiensis, as well as three separate species: Metapenaeopsis provocatoria longirostris, Metapenaeopsis quinquedentata and Metapenaeopsis velutina. The major pattern of relationships between all studied taxa of Metapenaeopsis was similar across all analytical methods in which species with one-valved petasma were genetically distinct from those with two-valved petasma. As expected from morphology, the remaining species with stridulating organ constituted a strongly supported clade. In contrast, a paraphyletic clade was resolved for species without stridulating organ which contradicts Crosnier's morphological classification scheme for Metapenaeopsis. Overall, the present molecular data indicated that the shape of petasma and stridulating organ were both phylogenetically significant morphological characters for this genus, adding further evidence for the Crosnier's proposal. |
| |
| Keywords: | DNA barcoding Phylogeny Phosphoenolpyruvate carboxykinase Sodium–potassium ATPase α-subunit |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
