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Genetic structure and diversity of green sea turtle (Chelonia mydas) from South China Sea inferred by mtDNA control region sequence
Institution:1. Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo, Kyoto 606-8502, Japan;2. Orido, Shimizu, Shizuoka, Shizuoka 424-0902, Japan;3. Sea Turtle Association of Japan, 5-17-18 Nagaomotomachi, Hirakata, Osaka 573-0163, Japan;4. Suma Aqualife Park Kobe, 1-3-5 Wakamiyacho Suma, Kobe, Hyogo 654-0049, Japan;5. Faculty of Biosphere–Geosphere Science, Okayama University of Science, 1-1 Ridai-mae Kita, Okayama, Okayama 700-0005, Japan;1. Fundação Pró Tamar, Rua Rubens Guelli, 134 sala 307, Salvador, Bahia, Brazil;2. North Carolina Wildlife Resources Commission, Beaufort, NC, United States;3. Centro Tamar/ICMbio, Av. Nossa Senhora dos Navegantes, 700-A — Vitória, Espirito Santo, Brazil;4. Department of Earth, Ocean and Atmospheric Science, Florida State University, Tallahassee, FL, United States
Abstract:We analyzed 88 control region sequences of green sea turtle (Chelonia mydas) from around Hainan Island in the South China Sea. These sequences had a length of 489 bp and revealed 8 mtDNA haplotypes of which four haplotypes (CMC1, CMC4, CMC7, and CMC8) had not been discovered before. Haplotype diversity (h) and nucleotide diversity (π) were 0.45 ± 0.054 and 0.0035 ± 0.0014, respectively. Neighbor-Joining tree based on control region sequences revealed that genetic relationship between green sea turtles from the South China Sea and from Japan Sea were very close. Clustering relationship based on control region sequences indicated that the South China Sea is an important breeding site and feeding habitat for green sea turtles, which connects with the Middle East Pacific, the Southwest Pacific and the Indian Ocean.
Keywords:MtDNA haplotypes  Control region  South China Sea  Biodiversity conversation  DNA sequencing
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