Isolation and characterisation of 26 microsatellite loci from a widespread tropical hydrozoan,Macrorhynchia phoenicea (Leptothecata,Aglaopheniidae), and cross-amplification in closely related species |
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| Institution: | 1. Université de La Réunion UMR ENTROPIE 9220, CS 92003, 97744 Saint Denis CEDEX 9, France;2. Laboratoire d’Excellence CORAIL, France;3. Aix Marseille Université, CNRS, IRD, Avignon Université, IMBE UMR 7263, 13397 Marseille, France;1. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;2. College of Pharmacy, Yanbian University, Yanji 133002, PR China;3. College of Pharmacy, Duksung Women''s University, 33, Samyang-ro 144-gil, Dobong-gu, Seoul 132-714, Republic of Korea;4. College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea;1. Institut Jean Lamour, UMR 7198 CNRS-Université Lorraine, Parc de Saurupt, 54011 Nancy, France;2. CRISMAT, UMR 6508 CNRS/ENSICAEN, LUSAC, Université de Caen Basse-Normandie, 6 Bd Maréchal Juin, 14050 CAEN Cedex 04, France;3. Institut Laue-Langevin (ILL), 6 rue J. Horowitz, BP 156-38042 Grenoble, Cedex 09, France;1. Applied Physics Department, University of Zaragoza, C/Pedro Cerbuna, 12, 50009 Zaragoza, Spain;2. Ariño Duglass, Pol. Ind. Royales Bajos, 50171, La Puebla de Alfindén, Spain;1. State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150080, China;2. The Fourth Hospital of Harbin Medical University, Harbin 150001, China;3. Sino-Russia Joint Lab for High Energy Beam, Shenyang Ligong University, Shenyang 110159, China;4. School of Automobile and Traffic Engineering, Heilongjiang Institute of Technology, Harbin 150050, China;5. Shanghai Key Laboratory of Advanced High-temperature Materials and Precision Forming, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, China;6. The State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, China;1. Mechanical & Aerospace Engineering, Oklahoma State University, Tulsa, 74106, OK, United States;2. Center for Nanoscale Materials, Argonne National Laboratory, Argonne, 60439, IL, United States |
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| Abstract: | We isolated and characterized 26 microsatellite loci for Macrorhynchia phoenicea (Busk, 1852), a rather common tropical hydrozoan from the Indo-Pacific. The number of alleles per locus ranged from 4 to 24. The observed heterozygosity ranged from 0.000 to 0.970 and the expected heterozygosity from 0.029 to 0.833. Ten loci were at Hardy–Weinberg equilibrium. No pair of loci presented linkage disequilibrium. Transferability of up to 18 loci was positive across four other Aglaopheniidae species from different genera. These loci will be used in studying reef population connectivity for these particular species at the scale of the Indo-Pacific, a promising but little explored research field. |
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| Keywords: | Aglaopheniidae Population genetics Cross-amplification Microsatellites Hydrozoan Connectivity |
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