Genetic diversity and structure of Pinus dabeshanensis revealed by expressed sequence tag-simple sequence repeat (EST-SSR) markers |
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| Institution: | 1. College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China;2. School of Life Science, Anqing Normal University, Anqing 246011, Anhui, China;3. The Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China;1. College of Life Sciences, Shaanxi Normal University, Xi''an 710062, China;2. National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi''an, Shaanxi 710062, China;3. Meteorological Bureau of Shaanxi Province, Xi''an 710014, China;1. Department of Biology Education, Seowon University, Cheongju 361-742, Republic of Korea;2. BioC, GReB, Botanic Institute of Barcelona (IBB-CSIC-ICUB), Passeig del Migdia s/n, Barcelona 08038, Spain;3. Department of Biology and the Research Institute of Natural Science, Gyeongsang National University, Jinju 660-701, Republic of Korea;1. State Key Laboratory of Earth Surface Processes and Resource Ecology and Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China;2. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;3. School of Mathematics and Computer Science, Northwest University for Nationalities, Lanzhou 730030, China;1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China;2. Guangzhou Academy of Forestry and Landscape Gardening, Guangzhou, 510405, China |
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| Abstract: | Assessing patterns of genetic variation in rare endangered species is critical for developing both in situ and ex situ conservation strategies. Pinus dabeshanensis Cheng et Law is an endangered species endemic to the Dabieshan Mountains of eastern China. To obtain fundamental information of genetic diversity, population history, effective population size, and gene flow in this species, we explored patterns of genetic variation of natural populations, in addition to an ex situ conserved population, using expressed sequence tag-simple sequence repeats (EST-SSR) markers. Our results revealed moderate levels of genetic diversity (e.g., HE = 0.458 vs. HE = 0.423) and a low level of genetic differentiation (FST = 0.028) among natural and conserved populations relative to other conifers. Both contemporary and historical migration rates among populations were high. Bayesian coalescent-based analyses suggested that 3 populations underwent reductions in population size ca. 10,000 yr ago, and that two populations may have experienced recent genetic bottlenecks under the TPM. Bayesian clustering revealed that individuals from the ex situ population were largely assigned to the ‘red’ cluster. Additionally, our results identified private alleles in the natural populations but not in the ex situ population, suggesting that the ex situ conserved population insufficiently represents the genetic diversity present in the species. Past decline in population size is likely to be due to Holocene climate change. Based on the genetic information obtained for P. dabeshanensis, we propose some suggestions for the conservation and efficient management of this endangered species. |
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| Keywords: | Genetic diversity Population history Contemporary and historical gene flow Effective population size Conservation |
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