Development and characterization of microsatellite markers for Vriesea simplex (Bromeliaceae) and cross-amplification in other species of Bromeliaceae |
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| Affiliation: | 1. Programa de Pós-graduação em Botânica, Museu Nacional, Universidade Federal do Rio de Janeiro, 20940-040 Rio de Janeiro, RJ, Brazil;2. Instituto de Biociências, Universidade de São Paulo, 05508090 São Paulo, SP, Brazil;3. Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil;4. Programa de Pós-Graduação em Ecologia e Biodiversidade, Universidade Estadual Paulista Julio Mesquita Filho, 13506-900 Rio Claro, SP, Brazil |
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| Abstract: | Ten polymorphic microsatellite loci were isolated and characterized for Vriesea simplex epiphytic and endemic species from the Brazilian Atlantic Rainforest. The number of alleles per locus ranged from 2 to 16. The observed and expected heterozygosity ranged from 0.000 to 0.414 and 0.068 to 0.795, respectively. All loci departed significantly from Hardy–Weinberg equilibrium. Linkage disequilibrium was not detected in any pair of loci. Transferability of 10 loci was positive across ten other Bromeliaceae species. The loci will be used for studying population genetic structure, mating system variation, and the origin and maintenance of reproductive barriers to gene exchange among sympatric Vriesea species. |
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| Keywords: | Atlantic Rainforest Cross-amplification Genetic structure Gene flow Microsatellite Bromeliad |
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