Tetranucleotide microsatellites for the barnacle Megabalanus coccopoma (Darwin, 1854) |
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| Affiliation: | 1. Nagasaki Prefectural Institute of Fisheries, Nagasaki 851-2213, Japan;2. Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki 852-8521, Japan;1. Faculty of Veterinary Medicine, Lusophone University of Humanities and Technologies, Lisbon, Portugal;2. Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal;3. Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, UTAD, 5001-911 Vila Real, Portugal;4. Animal and Veterinary Research Center (CECAV), School of Agrarian and Veterinary Sciences, UTAD, 5001-911 Vila Real, Portugal;5. Department of Veterinary Medical Imaging and Small Animal Orthopedics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;6. Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Mass Spectrometry Center, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;1. Laboratório de Evolução, Instituto de Estudos Costeiros, Universidade Federal do Pará, Alameda Leandro Ribeiro, s/n, Aldeia, 68.600-000, Bragança, PA, Brazil;2. Universidade Federal do Pará, Cametá, Trav. Padre Antônio Franco, 2617, Matinha, 68.400-000 Cametá, PA, Brazil;3. Institut de Ciències del Mar (ICM/CSIC), Passeig Marítim de la Barceloneta, 37-49. E-08003 Barcelona, Spain;4. Universidade Federal Rural da Amazônia, Campus de Capanema, Rua João Pessoa, 121.68700-030 Capanema, Pará, Brazil;5. CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Laboratório Associado, Campus Agrário de Vairão, Universidade do Porto, 4485-661 Vairão, Portugal |
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| Abstract: | The barnacle Megabalanus coccopoma is indigenous to the tropical Pacific Ocean, but was recently introduced to the coastal waters of the southeastern U.S.A. As part of a larger effort to investigate the population dynamics of this introduction, we designed 13 microsatellite primers specific to M. coccopoma and developed the accompanying polymerase chain reaction (PCR) conditions. We tested these primers on 42 individuals of M. coccopoma collected from two sampling locations in coastal Georgia, USA. The 13 loci developed showed means of 24.5 alleles per locus, 0.93 expected heterozygosity, 0.67 observed heterozygosity, and 0.91 polymorphic information content. The high variation observed within these microsatellite loci makes them useful tools for testing hypotheses related to population genetics, including source-sink dynamics for range expansions and rates of self-fertilization and outcrossing. |
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| Keywords: | Introduced species Non-native Microsatellite primers Invertebrate |
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