Characterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida,Brissidae) through Next Generation Sequencing |
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| Affiliation: | 1. Laboratoire de Biologie Marine (CP 160/15), Université Libre de Bruxelles (ULB), 50 avenue F. Roosevelt, 1050 Brussels, Belgium;2. Biogéosciences, UMR CNRS 6282, Université de Bourgogne, 6 boulevard Gabriel, 21000 Dijon, France;3. Smithsonian Tropical Research Institute, PO Box 0843-03092, Balboa, Panama;1. Department of Chemistry, Villanova University, Villanova, PA 19085, USA;2. Department of Food Science, Cornell University, Ithaca, NY 14853, USA;3. Department of Biodiversity Earth and Environmental Sciences, Drexel University, Philadelphia, PA 19104, USA;4. Department of Botany, Academy of Natural Sciences of Drexel University, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA;1. College of Life Sciences, Liaoning Normal University, Dalian 116081, China;2. Department of Biotechnology, Dalian Medical University, Dalian 116044, China;1. Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy;2. Department of Environmental and Life Science, Section Botany and Botanical Garden, University of Cagliari, Viale S.Ignazio, 13 I, 09123 Cagliari, Italy;3. Institute of Life Sciences – Scuola Superiore Sant''Anna, Via Santa Cecilia 3, 56127 Pisa, Italy;1. School of Marine Sciences, Ningbo University, Ningbo 315211, PR China;2. Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo 315100, PR China |
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| Abstract: | Eight microsatellite loci were characterized for Meoma ventricosa (Lamarck, 1816), a burrowing sea urchin that can be afflicted by a bacterial disease causing localized mass mortality. For the analyzed population (29 individuals from St. Croix, US Virgin Islands), we observed 8.125 mean number of alleles, 0.640 mean observed heterozygosity (Ho) and 0.747 mean expected heterozygosity (He). Two loci showed significant deviations from Hardy–Weinberg equilibrium. Overall, the described loci were characterized by a moderately high level of polymorphism suggesting that these markers are useful for a population genetic study in the Caribbean Sea. |
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| Keywords: | Microsatellites Echinoid Caribbean Tagged primer method 454 method |
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