Use of morphological traits and microsatellite markers to characterize the Tunisian cultivated and wild figs (Ficus carica L.) |
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| Affiliation: | 1. State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Institute of Sedimentary Geology, Chengdu University of Technology, Chengdu 610059, China;2. State Key Laboratory of Palaeobiology and Stratigraphy (Nanjing Institute of Geology and Palaeontology), CAS, Nanjing 210008, China;3. School of Earth Sciences and Key Laboratory of Mineral Resources in Western China (Gansu Province), Lanzhou University, Lanzhou 730000, China;4. School of Land and Resources, China West Normal University, Nanchong 637009, Sichuan, China;1. College of Fisheries, Jimei University, Xiamen 361021, PR China;2. Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Xiamen 361021, PR China;1. Institute of Forestry Ecology, Environment and Protection, and The Key Laboratory of Forest Ecology and Environment of State Forestry Administration, The Chinese Academy of Forestry, Beijing 100091, China;2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;3. Baiyunshan National Natural Reserve, Baojing 416500, China;1. Geriatric Unit, Lyon-Sud Hospital, Hospices Civils de Lyon, Lyon, France;2. Cardiology Department, European Society of Hypertension Excellence Center, Croix-Rousse Hospital and Lyon-Sud Hospital, Hospices Civils de Lyon, Lyon, France;3. Lyon University, CREATIS, CNRS UMR5220, INSERM U1044, INSA-Lyon, Université Claude Bernard Lyon 1, Lyon, France;4. Health Services and Performance Research (HESPER EA7425), Lyon, France;5. Oncology Unit, Lyon-Sud Hospital, Hospices Civils de Lyon, Lyon, France;6. Lyon University, CarMeN Laboratory, Inserm U1060, INRA U1397, Université Claude Bernard Lyon 1, INSA Lyon, Charles Mérieux Medical School, Oullins, France |
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| Abstract: | We used 8 morphological traits and 17 simple sequence repeats loci to characterize 71 cultivated and wild Tunisian fig trees (Ficus carica L.). Significant morphological differences were inferred from leaf traits. The statistical analysis showed two major fig groups that indicated a common morphological basis. A total of 74 SSR alleles was revealed, defining 63 unique multilocus genotypes indicating a substantial genetic diversity. Based on multilocus SSR genotypes an identification key was established using MFC30, MFC3, MFC11 and MFC19 loci to identify figs. Analysis of variance components of linkage disequilibrium shown that among 136 pairs of loci, 32 present a significant gametic disequilibrium. The parameter D′2IS (0.1284) was greater than D′2ST (0.0079), a pointer of close to zero variance in total simple, and consequently the more pronounced independence of the 17 SSR loci. The majority of Ohta's variance components of linkage disequilibrium followed a pattern caused by genetic drift or a non-systematic disequilibrium profiles and natural selection occurs only for LMFC24-MFC8 pair loci in cultivated figs. Our results suggest that the morphological and SSR markers are suitable to characterize figs and should be recommended in conservation management strategy. |
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| Keywords: | Wild figs Identification key Molecular marker Morphological traits SSRs Tunisia |
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