DNA barcoding and haplotyping in different species of Andrographis |
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| Institution: | 1. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan University of Nationalities, Kunming, Yunnan 650031, PR China;2. Kunming Xianghao Technology Co., Ltd., Kunming, Yunnan 650204, PR China;3. Department of Plant Resources Utilization, School of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, PR China;1. Department of Ornamental Horticulture, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, 311300 Lin''an, Hangzhou, China;2. Nurturing Station for State Key Laboratory of Subtropical Silviculture, 311300 Lin''an, Hangzhou, China;1. Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Northwest University, No. 229, Street Taibaibei, Xi''an 710069, Shaanxi Province, China;2. College of Life Sciences, Shaanxi Normal University, Xi''an 710119, China;1. Research Unit of DNA barcoding of Thai Medicinal Plants, Chulalongkorn University Drug and Health Products Innovation Promotion Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand;2. Omics Science and Bioinfomatics Center, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;3. Microbiome Research Unit for Probiotics in Food and Cosmetics, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;4. College of Pharmacy, Rangsit University, Pathumthani, 12000, Thailand;5. Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand;1. Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture & Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;2. Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China |
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| Abstract: | The genus Andrographis, belonging to the family Acanthaceae, contains several species of medicinal importance. Species, such as Andrographis alata, Andrographis echioides, Andrographis glandulosa, Andrographis lineata, Andrographis nallamalayana and Andrographis paniculata, with several bio-active compounds are being extensively used in folk medicine. However, difference of opinion exists with regard to inclusion of the species echioides into the genus Andrographis. The present study, using rbcL and matK sequences, for the first time established DNA barcodes for these six species. The nucleotide sequence of rbcL provided species-specific haplotypes for A. alata, A. lineata, and A. paniculata. Despite the differences with regard to nucleotide sequence, all the six species showed conserved amino acid sequence. However, all the six species showed distinct haplotypes in nucleotide sequence of matK and facilitated the identification and discrimination of these species. The phylogenetic tree generated with combined sequence of rbcL and matK revealed grouping of all the six species into a single clade confirming the positioning of the species echioides into the genus Andrographis. |
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| Keywords: | Acanthaceae DNA barcoding Haplotype Phylogenetic tree |
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