Evaluation of surfactants as solubilizing agents in microsomal metabolism reactions with lipophilic substrates |
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Authors: | Kathleen Randall Shun Wen Cheng Anne Therese Kotchevar |
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Institution: | (1) Department of Chemistry and Biochemistry, California State University, East Bay, Hayward, CA 94542, USA; |
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Abstract: | Solubilizing agents are routinely added when investigating the biotransformation of lipophilic substrates using hepatic microsomes.
For highly lipophilic compounds, the concentration of solvent or surfactant necessary for dissolution can be detrimental to
enzyme activity. This study evaluates the effect of 12 surfactants on microsomal metabolism and the ability of the same surfactants
to improve the aqueous solubility of the pentabrominated diphenyl ether BDE-100, a lipophilic environmental contaminant previously
found to be recalcitrant to in vitro metabolism. Of the surfactants investigated, Cremophor EL and Tween 80 displayed the
best combination of increased BDE-100 solubility and minimal inhibition of microsomal metabolism. However, a comparison of
the in vitro metabolism products of BDE-100 in the presence of the two surfactants revealed varying amounts of metabolites
depending on the surfactant used. |
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Keywords: | |
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