Microbial sensors for determination of aromatics and their chloro derivatives. Part III: Determination of chlorinated phenols using a biosensor containing Trichosporon beigelii (cutaneum) |
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| Authors: | K Riedel B Beyersdorf-Radeck B Neumann F Schaller |
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| Institution: | (1) Dr. Bruno Lange GmbH Berlin, Willstätter Str. 11, D-40549 Düsseldorf, Germany;(2) GBF Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany;(3) Max-Delbrück-Centrum of Molecular Medicine, Robert-Rössle Str. 10, D-13125 Berlin, Germany |
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| Abstract: | An amperometric biosensor for the determination of phenol and chlorophenols using Trichosporon beigelii (cutaneum) has been developed. This sensor is more sensitive to chlorophenols, especially to mono- and dichlorinated phenols, than to phenol and is insensitive to benzoate. A linear relationship between the current range and the concentration of 4-chlorophenol is observed up to 40  mol/l. The detection limit for all substrates studies is 2 mol/l. the current difference is reproducible within 5.5% when the test solution contains 0.40 mol 4-chlorophenol. |
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