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Genetic location ofrra mutation and its role in alleviation of rgl restriction
Authors:Dr P Kannan  K Dharmalingam
Institution:(1) Present address: Department of Tumor Biology, The University of Texas, M.D. Anderson Cancer Center, 1515 Holecombe Blvd., 77030 Houston, TX, USA;(2) Department of Biotechnology, School of Biological Sciences, Madurai Kamaraj University, 625 021 Madurai, India
Abstract:Restriction of glucosyl-free HMC-DNA mediated by RglB is alleviated inrecBC sbcA strains ofEscherichia coli K12. Mutation in the unlinkedrra gene reverses thisrecBC sbcA-mediated alleviation. The map position ofrra is 90.16 min on the standard map, and therra + gene product counteracts Rgl restriction. The activation of therra gene is controlled by thesbcA gene, and this regulation does not seem to require the involvement of other gene functions.
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