Pteridine-dependent oxygen activation in neutrophils |
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Authors: | M G Petukh G N Semenkova D Fuchs S N Cherenkevich |
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Institution: | 1. Department of Biophysics, Belarussian State University, Minsk, Belarus 2. Medical University, Biocenter, Innsbruck, Austria
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Abstract: | We investigated the influence of neopterin and 7,8-dihydroneopterin on the myeloperoxidase activity and secretory degranulation
in neutrophils and interaction of pteridines with its major substrate (hydrogen peroxide) and intermediate product of halogenation
cycle (hypochlorous acid). It was shown that, in neutrophils, the redox-pair, neopterin and 7,8-dihydroneopterin, control
oxygen activation, which is regulated by myeloperoxidase. Pteridines influence the secretion of myeloperoxidase depending
on concentration and decrease the level of hydrogen peroxide and hypochlorous acid, which are the substrate and intermediate
product of the enzyme, respectively. It was found that, in micromolar concentrations, 7,8-dihydroneopterin is a noncompetitive
inhibitor of myeloperoxidase. We suppose that myeloperoxidase facilitates 7,8-dihydroneopterin oxidation by hypochlorous acid
and results in an increase in neopterin concentration. These changes modify the concentration of intracellular and extracellular
reactive oxygen species. |
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Keywords: | |
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