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A rapid and specific enrichment procedure forHyphomicrobium spp.
Authors:Margaret M Attwood  W Harder
Institution:(1) Department of Microbiology, University of Groningen, Kerklaan 30, Haren (Gr.), the Netherlands;(2) Present address: Department of Microbiology, University of Sheffield, England
Abstract:An enrichment procedure for the isolation of stalked bacteria of the genusHyphomicrobium is described. The method is based on the use of an organic C1 compound as carbon and energy source for growth together with anaerobic incubation in the presence of nitrate as an electron acceptor. Optimal conditions for the growth of a number ofHyphomicrobium isolates have been investigated. Applying these conditions,Hyphomicrobium spp. have been enriched from a wide range of natural habitats within 1–2 weeks. This work was supported by a Grant from the Medical Research Fund, University of Sheffield, England, to M.M.A.
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