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The stability of the lactose and citrate plasmids in Lactococcus lactis subsp. lactis biovar. diacetylactis
Authors:Mark R Smith  Jeroen Hugenholtz  Péter Mikóczi  Ellen de  Ree  Alan W Bunch  Jan AM de  Bont
Institution:Laboratory for Electron Microscopy, Biological Centre, University of Groningen, Haren, Netherlands.
Abstract:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a narrow zone between the crystalloid and the peroxisomal membrane. In non-crystalline organelles the enzyme was present throughout the peroxisomal matrix. Other peroxisomal matrix enzymes studied for comparison, namely dihydroxyacetone synthase, amine oxidase and malate synthase, all were present throughout the AO crystalloid. The advantage of location of catalase at the edges of the AO crystalloids for growth of the organism on methanol is discussed.
Keywords:Peroxisomes  Alcohol oxidase  Crystalloids  Catalase  Immunocytochemistry              Hansenula polymorpha
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