High resolution derivative denaturation profiles of DNA in the presence of copper(II) ions

Derivative denaturation profiles of calf thymus DNA in the presence of copper(II) ions have been directly obtained from high resolution thermal denaturation profiles recorded in an isoabsorbance wavelength of the AT and GC hyperchromic spectra. The analysis of the very sensitive profiles provides further evidence that the melting temperature (Tm) of DNA decreases in the presence of stoichiometric ratio of copper(II) ions to nucleotide. Also, evidence is given of peculiar behaviour at higher temperatures where a new melting transition is observed. This phenomenon could be in line with the presence of bridging of DNA single strands by copper ions which are disrupted when the temperature is raised.