ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae |
| |
Authors: | T Drgon L Sabová N Nelson J Kolarov |
| |
Institution: | Cancer Research Institute, Slovak Academy of Sciences, Bratislava. |
| |
Abstract: | All three genes (AAC1, AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication. |
| |
Keywords: | |
|
|