An H-2K gene of the t
w32mutant at the T/t complex is a close parent of an H-2K
qgene |
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Authors: | Takashi Morita Christiane Delarbre Michel Kress Philippe Kourilsky Gabriel Gachelin |
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Institution: | (1) Unité de Biologie Moléculaire du Gène, U. 277 I.N.S.E.R.M. and E.R. C.N.R.S. No. 201, Institut Pasteur, 25, rue du Dr. Roux, 75724 Paris Cédex 15, France;(2) Laboratoire d'Oncologie Moléculaire, I.R.S.C., B.P. No. 8, 94802 Villejuif Cédex, France;(3) Present address: Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-Oka, Suita Osaka, Japan |
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Abstract: | Two recombinant mice have been recovered from the progeny of Ttf/t
w32+ animals. They have lost the tw32 lethality factor(s) and gained tufted, presumably from the T chromosome. Southern blot analysis of class I genes of these two new partial t
PA027 and t
PA286 haplotypes indicates that they have retained at least part of the major histocompatibility complex of the t
w32 chromosome (H-2 haplotype H-2
w28). We have prepared a phage library of Eco RI-digested DNA from homozygous t
PA027 animals. Upon screening the library with a cDNA probe specific for H-2K genes, we isolated a class I gene displaying all of the distinctive features of a genuine H-2K gene, and which could thus be defined as an H-2K
w28 gene. The H-2K
w28 gene is 92–95% homologous to H-2K
band H-2K
dgenes and differs significantly from the other class I genes sequenced so far. Homology with the H-2K
bsequence reaches nearly 100% in the 3 part of the H-2K
w28 gene. Moreover, the homology with an H-2K
qcDNA sequence reaches 99.8%. Several hypotheses can account for the near identity of H-2K
b, H-2K
q,and H-2K
w28 gene sequences: either recombination between H-2
w28 and H-2
band H-2
qsequences occurred before or at the.time the strain was established, or the class I genes of the t
w32 chromosome and the H-2
band H-2
qgenes found in inbred strains of mice have separated from each other rather recently. |
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Keywords: | |
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