In vitro effects of monoterpenes on chloroplast membranes |
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Authors: | Hanjo Klingler Silvi Frosch Edgar Wagner |
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Affiliation: | (1) Biologisches Institut II (Botanik), Universität Freiburg i. Br., Schänzlestrasse 1, 7800 Freiburg, Germany |
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Abstract: | A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-dependent degradation of envelope membranes could be prevented by addition of -tocopherol prior to monoterpene incubation.Photosynthetic electron transport of photosystem II was completely inhibited by -pinene, Triton X-100 and linolenic acid. Inhibition could be modulated by addition of -tocopherol or lecithin (phosphatidylcholine) either before or after inhibition by monoterpenes and detergent.Percentage reconstitution of photosynthetic electron transport inhibited by -pinene depended on light conditions and incubation time. |
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Keywords: | photosynthetic electron transport structure and function relationship lipids /content/l84lr32h42lx4480/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >-tocopherol free-flow electrophoresis |
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