The Relationship Between Taurine and 3-Nitrotyrosine Level of Hepatocytes in Experimental Endotoxemia |
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| Authors: | Hüsamettin Erdamar Nurten Türközkan Barboros Balabanli Gonca Ozan Filiz Sezen Bircan |
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| Institution: | (1) Department of Biochemistry, Beytepe Military Hospital, Incek, Ankara, Turkey;(2) Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey;(3) Department of Biology, Faculty of Arts and Science, Gazi University, 06500 Teknikokullar, Ankara, Turkey |
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| Abstract: | It has been proposed that taurine may function as an oxidant in a dose-dependent manner in vivo and in vitro. The present
study was carried out to investigate the relationship between taurine concentration and 3-nitrotyrosine level, a stable marker
of peroxynitrite action, in hepatocytes of guinea pig in endotoxemia before and after taurine administration. The levels of
taurine and 3-nitrotyrosine were measured by HPLC method. In the present study, taurine was low concentration in hepatocytes
exposed to endotoxemia. In taurine plus endotoxin treated animals, HPLC analysis showed higher taurine level compared with
animals only supplemented with taurine. But 3-nitrotyrosine levels were same in both taurine alone and taurine plus endotoxin
groups. In conclusion, taurine is able to prevent the damaging effect of peroxynitrite, at concentration measured in hepatocytes,
in our experimental conditions. |
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| Keywords: | Taurine Peroxynitrite 3-Nitrotyrosine Endotoxin Hepatocyte High performance liquid chromatography |
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